Adipose Tissue Core
Excess adipose tissue is the defining characteristic of obesity. Adipose tissue distribution, morphology, metabolic and secretory characteristics, and macrophage infiltration/inflammation are contributing factors not only in the onset and maintenance of obesity but also of diabetes, hypertension, dyslipidemia, and cardiovascular diseases. The purpose of the Adipose Tissue Core (AT Core) is to provide an efficient and cost-effective means to assist investigators in need of morphometric and functional characterization of adipose tissue in their studies, and to promote interactions and collaborations between investigators who share interests in adipose tissue biology.
The AT Core provides training and services in the following areas:
1) Determination of gene expression, activity, and protein levels for adipokines, insulin signal transduction elements, and other adipose tissue-associated proteins.
2) Isolation of adipocytes, macrophages, and other components of the stromal vascular fraction from whole adipose tissues.
3) Measurements of adipose tissue morphology and cellularity.
4) Measurement of metabolic, biochemical, and endocrine functions of whole adipose tissue and isolated adipocytes.
5) Instructions to students, postdoctoral fellows, and investigators in measuring adipocyte size, macrophage infiltration, and in performing whole body adipose tissue depot dissection. Training in additional techniques is also possible.
The allocation of services is made based on following criteria: access of these services will support existing NIH funded research; the services are likely to lead to the acquisition of data needed to apply for independent research funding; the services will advance knowledge in the field of obesity research and/or the services provide an educational venue for students. Services and training are rendered on the first come first serve basis with the NYONRC members having the priority over non-members in scheduling and pricing. If you would like to use the AT Core services, please complete the online Service Request Form and email the completed form to the Core Directors.
Yiying Zhang, Ph.D.
Tony Ferrante, M.D., Ph.D.
Joseph Vasselli, Ph.D.
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